Breeding for cytoplasmic male sterility in (Brassica oleracea L. var. italica Plenck)

Abstract

A male sterile line of kale EC 173419 with Ogura cytoplasm was crossed in 1990-91 and back crossed in 1991-92 with Green Sprouting Broccoli (GSB) to transfer male sterile cytoplasm into broccoli (Brassica oleracea L. var. italica Plenck) at JARI Regional Station, Katrain, located in Kullu valley, Himachal Pradesh at an altitude of 1500 m. Backcrossing was not attempted during 1992-93 and 1993-1994. However, in 1994-95 and 1995-96 because of poor quality of GSB, it was substituted by another breeding line 005559 as recurrent parent. Since kale has chilling requirement for flowering a male sterile back cross progeny (kale EC 173419 x GSB2 x 0055592) from Katrain was used as source of male sterile cytoplasm.