Identification of S haplotypes in doubled haploid lines of non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino)

Abstract

A set of 24 Doubled haploid (DH) lines of non-heading Chinese cabbage obtained through microspore culture were used for identification of self-incompatibility (SI) and S haplotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis with S-locus receptor kinase (SRK)-specific primers. The DH lines were grouped into five S haplotypes. The SI of two of these haplotypes was strong, whereas that of the remaining haplotypes was weak. The results obtained were consistent with the self-compatibility indices found. PCR-RFLP analysis using DH lines could enhance selection efficiency and improve the application of SI in the hybrid breeding of non-heading Chinese cabbage.