Molecular profiling of rice (Oryza sativa L.) genotypes differing in micronutrients (iron and zinc) content

Abstract

The present study was undertaken to study the genetic diversity among fourteen rice genotypes, micronutrient rich indica, Basmati, japonica and commercial cultivars using 50 microsatellite markers uniformly distributed across the rice genome. A total of 257 alleles were detected that ranged from 1 to 9 with an average of 5.14 alleles per locus. The overall size of amplified PCR products ranged from 73 to 585 bp. The Polymorphic Information Content (PIC) value ranged from 0 to 0.862 with an average of 0.66. The NTSYSpc UPGMA tree cluster as well as WINBOOT analysis placed the Fe-rich genotypes with Zn-rich Jaya and high-yielding indica rice genotypes, Fe-rich Taraori Basmati in Basmati rice group with Pusa Basmati 1 and Basmati 370, zinc rich TNG67 in japonica group. The profiting of the genotypes would pave the way for genetic improvement for micronutrients in rice.