Chromosome identification and karyotype analysis of rice bean [Vigna umbellata (Thunb.) Ohwi et Ohashi] using fluorescence in situ hybridization
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Abstract
Rice bean (Vigna umbellata), an underutilized climate-resilient legume, includes the high-yielding, leaf spot resistance variety VRB-3 (Him Shakti). In the present study, using fluorescence in situ hybridization, one pair each of 45S and 5S rRNA gene loci was localized to the terminal region of the chromosomes and the estimated genome content for VRB-3 is 1.35pg against the reference standard Solanum lycopersicum cv. Stupicke.
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