DNA content, ploidy level determination and genetic variations of okra (Abelmoschus esculentus L.) genotypes
Main Article Content
Abstract
Okra (Abelmoschus esculentus L.) is a minor small crop in Jordan; it has attracted a lot of attention as a substitute for conventional
vegetables throughout the world. There are conflicting reports about chromosome numbers in this species. To determine the ploidy
level of different okra genotypes, okra root tips were treated with HCl maceration, enzymatic maceration, and Carmine acid squashing.
Treating cells with HCl didn’t macerate the cell in a way that enables chromosome count. The enzymatic treatment combination
showed no significant effect on cell maceration. Carmine’s acetic acid squashing method was able to digest the cells but in a way that
all chromosomes from neighboring cells gathered, making it difficult to count them from each cell. Flow cytometry as an alternative
way to assess okra ploidy, was considered as an option. The genome size of okra ranged from 4.11 pg 2C in genotype 43 to 6.27 pg 2C
in genotype 30.
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