Validation of known ToLCV markers associated with ToLCV resistance in tomato through Bulked Segregant Analysis

Abstract

After phenotyping of the segregating F2 population derived from a highly resistant ToLCV line of wild tomato IIHR 2101 (S. habrochaites) and a susceptible cultivated line AT-3 (S. lycopersicum), the ratio of resistant to susceptible tomato plants was found to be approximately 3:1. In the correlation analysis phenol showed a significant and negative correlation (r = -0.8166) with the disease incidence. Total 235 plants from F2 segregating generation were subjected to molecular marker analysis employing a Bulked Segregant Analysis (BSA) approach. Out of 146 primers surveyed for parental screening, only two were found able to discriminate both the parents (highly resistant and highly susceptible) sufficiently. Two markers (Sp and C2_At5g51110) were identified as linked to ToLCV resistance gene through BSA.