INDUCTION OF POLLEN EMBRYOGENESIS AND CYTOLOGICAL VARIABILITY IN ARACHIS HYPOGAEA L. THROUGH ANTHER CULTURE

Abstract

Regeneration of haploids by tissue culture in groundnut would facilitate the rapid production of homozygous lines. Response of genotypes to different media was studied. Murashige and Skoog [1] medium supplemented with 3% sucrose or coconut milk, gave the best callus proliferation and rhizogenesis. Variable chromosome numbers, ranging from n=20 to n=40, were observed in the callus cells. The responding anthers revealed early segmentation of pollen, multicellular pollen and pollen embryoin all the three genotypes.