Genetic variation in gamma ray induced mutants in blackgram as revealed by RAPD and ISSR markers

Abstract

Random amplifi~d polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in 12 gamma ray induced morphotoawal mutants of blackgram [Vigna mungo (L.) Hepper]. Of the 35 random and eight ISSR primers used, 15 random and five ISSR primers detected polymorphism among the mutants. Total number of bands varied from 2 to i for RAPD and from 6 to 10 for ISSA. Th~ number of polymorphic bands varied from 1 to 3 for RAl?D and from 1 to ii for ISSR. Percentage of polymorphism ranged from 12.5 to 50.0 for RAPD and 12.5 to 44.4 for ISSR. ~gnif1cant DNA polymorphism amon9 the mutants were observed using RAPD (25.8%) and ISSR (33.3%) markers. An young leaf chlor,na mutant and a smooth pod mutant showed more DNA polymorphism as compared to the parent. DNA polymorphism data revealed by RAPD and ISSR could facilita,te selection of mutants to be involved in cross breeding and g6{lome mapping.