A modified DNA isolation protocol for high-quality DNA and long-term storability in grasspea (Lathyrus sativus L.)
Main Article Content
Abstract
Grasspeas pose challenges in obtaining high-quality DNA due to their seed and leaves’ high protein, phenols, secondary metabolites, and neurotoxins. This study investigated the challenges faced by the CTAB method in DNA isolation, subsequent enhancements in quality and storage with a modified CTAB approach using triton, PVP, high salt TE and sodium acetate to extract the high-quality genomic DNA from grasspea.
Article Details
How to Cite
Ramya, K. R. ., Gupta, R. ., Mishra, M. ., Pandey, A. ., Tripathi, K. ., Bhatt, K. C. ., & Singh, R. . (2023). A modified DNA isolation protocol for high-quality DNA and long-term storability in grasspea (Lathyrus sativus L.). INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 83(04), 602–604. https://doi.org/10.31742/ISGPB.83.4.16
Section
Short Research Article
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.