Immature ovule embryo culture - A tool to mUltiply wild cotton (Gossypium L.) germplasm

Abstract

Germplasm collection and maintenance is the ideal to obtain seed for all species but seed is further frequently incompletely ripened, inviable/ not found. Supplemental use of in vitro techniques of cotton germplasm conservation have been proposed in order to maximize efforts to preserve our genetic; resources of cotton germplasm [1-2]. The present investigation was carried out with an aim to (1) determine appropriate conditions for growing young immature ovules of Gossypium spp. through in vitro culture techniques, (2) utilize this information for multiplication of plant of different species, of which multiplication through seed is rather difficult; and (3) to make available wild species and entire gene pool of genes of Gossypium to cotton geneticist and breeders for their utilization.