Molecular analysis of mitochondrial DNA of lines representing a specific CMS-fertility-restorer system of pearl millet [Pennisetum glaucum (L.) R. Sr.] by RAPD markers

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A. A. Kale
S. V. Munjal

Abstract

Molecular characterization of the mitochondrial DNA [mtDNA] from the etiolated pearl millet [Penn/setum glsucum (L.) R. Br.] seedlings of cytoplasmic male sterile (CMS) line having A1 cytoplasm alongwlth Its maintainer, restorer and restored lines of hybrid RHRBH-8609 (Shrsdhs) revealed polymorphism by 10 RAPD primers. Only four primers showed polymorphism between a CMS, RHRB-1A and Its fertile Isonuclear line, RHRB·1 B. However, 5 primers showed polymorphism between the CMS line and its restorer line, RHRB-138R. The primers OPC15, OPC16 and OPG13 generated fragments of -3.90 kb, 1.59 kb and 1.02 kb size only In the mtDNA of a male sterile line which might be associated with the CMS phenotype of RHRB-1A in pearl millet.

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How to Cite
Kale, A. A., & Munjal, S. V. (2005). Molecular analysis of mitochondrial DNA of lines representing a specific CMS-fertility-restorer system of pearl millet [Pennisetum glaucum (L.) R. Sr.] by RAPD markers. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 65(01), 1–4. https://doi.org/.
Section
Research Article