Genetic analysis of wild species in support of evolutionary changes of the genus Gossypium through ISSR markers

Abstract

Twenty-two wild species of Gossypium belonging to seven genomic groups were studied using Inter Simple Sequence Repeat (ISSR) markers to establish phylogenetic relationship within the genus. Among 25 ISSR primers used, 19 were found to be scorable on agarose gel of which 88% were polymorphic. Genome specific and unique markers were observed in this study. In support of the evolutionary study, 3 major clusters were found in the dendrogram, one composed of A and B genome species and the other composed of D, AD and C genome species. E, F and G genome species were placed out side the major cluster at the end of dendrogram. These results suggested that ISSR-PCR markers are potentially useful in establishing genetic relationship, genome specificity and genetic basis of evolution among the wild species of genus Gossypium.