Transfer of GA-sensitive dwarfing gene Rht18 by marker assisted breeding in HD3086 (Triticum aestivum L.) and multi-environment trials under varying seedling emergence regimes
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Abstract
The height reduction caused by the prevalent semi-dwarfing GA-insensitive gene Rht-B1b is associated with numerous negative pleiotropic effects, the major ones being reduced coleoptile lengths and lower early establishment rates, especially in moisture-stressed environments worldwide. Hence, breeding for wheat varieties with alternate GA-responsive (GAR) semi-dwarfing genes, such as Rht18, is an efficient approach for developing varieties for the moisture-stressed regimes globally. In this study, a structured marker-assisted backcross (MABC) breeding program was conducted to transfer the Rht18 gene into the mega wheat variety, HD3086. A total of 46 Rht18 positive BC2F5 lines were identified with an average background genome recovery of 88.34% using Wheat Breeder’s 35K Axiom SNP Array. Multi-environment trials were carried out at three locations under two conditions, i.e., stubble retained (SR) and rainfed (RF). ANOVA revealed significant differences for most of the traits in these trials. Plant height (PH) reduction in Rht18 introgressed lines ranged from 8.25–12.97 cm under SR and 5.25–10.15 cm under RF regimes. GGE biplots indicated specifically adapted and stable genotypes for yield in stubble-retained and rainfed regimes. These Rht18 lines hold strong potential for varietal release with further multi-location and multi-season trials. Ultimately, they will contribute to broadening the genetic base of Rht genes, especially for improving wheat adaptation in moisture-stressed environments.
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References
Ahmadi, J., Vaezi, B. and Hossein Fotokian M. 2012. Graphical analysis of multi-environment trials for barley yield using AMMI and GGE-biplot under rain-fed conditions. J plant physiol breed., 2: 43-54.
Alghabari, F., Ihsan, M.Z., Hussain, S., Aishia, G. and Daur, I. 2015. Effect of Rht alleles on wheat grain yield and quality under high temperature and drought stress during booting and anthesis. Environ Sci Pollut Res. 22: 15506-15515.
Aravind, J., Wankhede, D.P., Kaur, V. and Aravind, M.J. 2021. Package ‘augmentedRCBD’.
Balyan, H.S. and Lohia, R.S. 1998. Pleiotropic effects of Rht dwarfing genes on grain yield and its component traits in wheat under rainfed environment. Indian J Genet. 58: 169-176.
Bellundagi, A., Ramya, K.T., Krishna, H., Jain, N., Shashikumara, P., Singh, P.K., Singh, G.P. and Prabhu, K.V. 2022. Marker-assisted backcross breeding for heat tolerance in bread wheat (Triticum aestivum L.). Front Genet. 13: p.1056783.
Berry, P.M. and Spink, J. 2012. Predicting yield losses caused by lodging in wheat. Field Crops Res. 137: 19-26.
Bhagwat, S.G. and Bhatia, C.R. 1994. Quantitative differences in the gibberellin induced alpha amylase activity from aleurone layers of tall and semi-dwarf wheat cultivars. Cereal Res Commun. 12: 129-134.
Borojevic, K. and Borojevic, K. 2005. The transfer and history of “reduced height genes”(Rht) in wheat from Japan to Europe. J Hered. 96: 455-459.
Botwright, T., Rebetzke, G., Condon, T. and Richards, R. 2001. The effect of rht genotype and temperature on coleoptile growth and dry matter partitioning in young wheat seedlings. Funct Plant Biol. 28: 417-423.
Botwright, T.L., Rebetzke, G.J., Condon, A.G. and Richards, R.A. 2005. Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.). Ann Bot. 95: 631-639.
Chen, L., Hao, L., Condon, A.G. and Hu, Y.G. 2014. Exogenous GA3 application can compensate the morphogenetic effects of the GA-responsive dwarfing gene Rht12 in bread wheat. PloS one. 9: p.e86431.
Derkx, A.P., Harding, C.A., Miraghazadeh, A. and Chandler, P.M. 2017. Overgrowth (Della) mutants of wheat: development, growth and yield of intragenic suppressors of the Rht-B1c dwarfing gene. Funct Plant Biol. 44: 525-537.
Dharmaraj, D., Selvaraj, R., Kari, B., Govindan, S., Muthurajan, R. and Paramasiwam, J. 2024. Marker-assisted pseudo-backcrossing for developing climate-resilient rice. Sci Rep. 14: 1-16.
Doyle, J.J. and Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical bulletin.
Eagles, H.A., Cane, K., Trevaskis, B., Vallance, N., Eastwood, R.F., Gororo, N.N., Kuchel, H. and Martin, P.J. 2014. Ppd1, Vrn1, ALMT1 and Rht genes and their effects on grain yield in lower rainfall environments in southern Australia. Crop Pasture Sci. 65: 159-170.
Ellis, M., Spielmeyer, W., Gale, K., Rebetzke, G. and Richards, R. 2002. " Perfect" markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet. 105: 1038-1042.
Ellis, M.H., Rebetzke, G.J., Chandler, P., Bonnett, D., Spielmeyer, W. and Richards, R.A. 2004. The effect of different height reducing genes on the early growth of wheat. Funct Plant Biol. 31 .583-589.
Enghiad, A., Ufer, D., Countryman, A.M. and Thilmany, D.D. 2017. An overview of global wheat market fundamentals in an era of climate concerns. Int J Agron. 61 .58-65.
Foulkes, M.J., Slafer, G.A., Davies, W.J., Berry, P.M., Sylvester-Bradley, R., Martre, P., Calderini, D.F., Griffiths, S. and Reynolds, M.P. 2011. Raising yield potential of wheat. III. Optimizing partitioning to grain while maintaining lodging resistance. J Exp Bot. 62: 469-486.
Gale, M.D., Youssefian, S. and Russell, G.E. 1985. Dwarfing genes in wheat. Prog plant breed. 1: 1-35.
Giraldo, P., Royo, C., González, M., Carrillo, J.M. and Ruiz, M. 2016. Genetic diversity and association mapping for agromorphological and grain quality traits of a structured collection of durum wheat landraces including subsp. durum, turgidum and diccocon. PloS one. 11: p.e0166577.
Golfam, R., Kiarostami, K., Lohrasebi, T., Hasrak, S. and Razavi, K. 2021. A review of drought stress on wheat (Triticum aestivum L.) starch. Farming Manage. 6: 47-57.
Gollin, D., Hansen, C.W. and Wingender, A.M. 2021. Two blades of grass: The impact of the green revolution. J Pol Econ. 129: 2344-2384.
Grant, N.P., Mohan, A., Sandhu, D. and Gill, K.S. 2018. Inheritance and genetic mapping of the reduced height (Rht18) gene in wheat. Plants. 7: 58.
Gupta, P.K., Langridge, P. and Mir, R.R. 2010. Marker-assisted wheat breeding: present status and future possibilities. Mol Breed. 26: 145-161.
Haque, M., Martinek, P., Watanabe, N. and Kuboyama, T. 2011. Genetic mapping of gibberellic acid-sensitive genes for semi-dwarfism in durum wheat. Cereal Res Commun. 39: 171-178.
Hoyos‐Villegas, V., Wright, E.M. and Kelly, J.D. 2016. GGE biplot analysis of yield associations with root traits in a Mesoamerican bean diversity panel. Crop Sci. 56: 1081-1094.
Huang, T., Yang, H., Huang, C. and Ju, X. 2017. Effect of fertilizer N rates and straw management on yield-scaled nitrous oxide emissions in a maize-wheat double cropping system. Field Crops Res. 204: 1-11.
Jobson, E.M., Johnston, R.E., Oiestad, A.J., Martin, J.M. and Giroux, M.J. 2019. The impact of the wheat Rht-B1b semi-dwarfing allele on photosynthesis and seed development under field conditions. Front Plant Sci. 10: 388208.
Joshi, A.K., Mishra, B., Chatrath, R., Ortiz Ferrara, G. and Singh, R.P. 2007. Wheat improvement in India: present status, emerging challenges and future prospects. Euphytica. 157: 431-446.
Khalid, M.A., Ali, Z., Tahir, M.H.N., Ghaffar, A. and Ahmad, J. 2023. Genetic effects of GA-responsive dwarfing gene Rht13 on plant height, peduncle length, internodal length and grain yield of wheat under drought stress. Genes. 14: 699.
Khan, H., Chatrath, R., Kumar, S., Mishra, C.N., Gupta, V., Mamrutha, H.M., Jasrotia, P., Bhardwaj, S.C., Kashyap, P.L., Gangwar, O.P. and Kumar, R. 2020. Development and evaluation of high yielding, multiple disease resistant bread wheat variety-Karan Vandana (DBW187). J Cereal Res. 12: 420-436.
Kumar, P.K., Bellundagi, A., Krishna, H., Mallikarjuna, M.G., Thimmappa, R.K., Rai, N., Shashikumara, P., Sinha, N., Jain, N., Singh, P.K. and Singh, G.P. 2023. Development of bread wheat (Triticum aestivum L) variety HD3411 following marker-assisted backcross breeding for drought tolerance. Front Genet. 14: 1046624.
Kumar, V., Kumar, M., Singh, S.K. and Chandra, S.K. 2015. Impact of conservation agriculture on yield, nutrient uptake and quality of wheat crop in calciorthent. Plant Arch. 15: 371-376.
Liu, Y., Zhang, J., Hu, Y.G. and Chen, J. 2017. Dwarfing genes Rht4 and Rht-B1b affect plant height and key agronomic traits in common wheat under two water regimes. Field Crops Res. 204: 242-248.
Maccaferri, M., Sanguineti, M.C., Mantovani, P., Demontis, A., Massi, A., Ammar, K., Kolmer, J.A., Czembor, J.H., Ezrati, S. and Tuberosa, R. 2010. Association mapping of leaf rust response in durum wheat. Mol Breed. 26: 189-228.
Mallick, N., Vinod, Sharma, J.B., Tomar, R.S., Sivasamy, M. and Prabhu, K.V. 2015. Marker‐assisted backcross breeding to combine multiple rust resistance in wheat. Plant Breed. 134:172-177.
Mérida-García, R., Bentley, A.R., Gálvez, S., Dorado, G., Solís, I., Ammar, K. and Hernandez, P. 2020. Mapping agronomic and quality traits in elite durum wheat lines under differing water regimes. Agronomy. 10: p.144.
Murchie, E.H. and Burgess, A.J. 2022. Casting light on the architecture of crop yield. Crop Environt. 1: 74-85.
Nelson, S.K. and Steber, C.M. 2016. Gibberellin hormone signal perception: down‐regulating DELLA repressors of plant growth and development. Annu Plant Rev. 49: 153-188.
Nonaka, S. 1995. Breeding of semi-dwarf wheat. Rec Adv Plant Breed. 27: 69-79.
Olivoto, T. and Lúcio, A.D.C. 2020. metan: An R package for multi‐environment trial analysis. Methods Ecol Evol. 11: 783-789.
Ortiz, R., Sayre, K.D., Govaerts, B., Gupta, R., Subbarao, G.V., Ban, T., Hodson, D., Dixon, J.M., Ortiz-Monasterio, J.I. and Reynolds, M. 2008. Climate change: can wheat beat the heat?. Agric Ecosyst Environt. 126: 46-58.
Pask, A., Joshi, A.K., Manès, Y., Sharma, I., Chatrath, R., Singh, G.P., Sohu, V.S., Mavi, G.S., Sakuru, V.S.P., Kalappanavar, I.K. and Mishra, V.K. 2014. A wheat phenotyping network to incorporate physiological traits for climate change in South Asia. Field Crops Res. 168: 156-167.
Peng, J., Richards, D.E., Hartley, N.M., Murphy, G.P., Devos, K.M., Flintham, J.E., Beales, J., Fish, L.J., Worland, A.J., Pelica, F. and Sudhakar, D. 1999. ‘Green revolution’genes encode mutant gibberellin response modulators. Nature. 400: 256-261.
Ranjan, R., Yadav, R., Gaikwad, K., Kumar, M., Kumar, N., Babu, P., Pandey, R. and Joshi, A.K. 2021. Genetic variability for root traits and its role in adaptation under conservation agriculture in spring wheat. Indian J Genet. 81: 24-33.
Rebetzke, G.J.; Richards, R.A.; Fischer, V.M. and Mickelson, B.J. 1999. Breeding long coleoptile, reduced height wheats. Euphytica. 106: pp.159-168.
Rebetzke, G.J. and Richards, R.A. 2000. Gibberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat. Aust J Agric Res. 51: 235-246.
Rebetzke, G.J., Bruce, S.E. and Kirkegaard, J.A. 2005. Longer coleoptiles improve emergence through crop residues to increase seedling number and biomass in wheat (Triticum aestivum L.). Plant Soil`. 272: 87-100.
Rebetzke, G.J., Ellis, M.H., Bonnett, D.G., Mickelson, B., Condon, A.G. and Richards, R.A. 2012. Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.). Field Crops Res. 126: 87-96.
Rebetzke, G.J., Kirkegaard, J.A., Watt, M. and Richards, R.A. 2014. Genetically vigorous wheat genotypes maintain superior early growth in no-till soils. Plant Soil. 377: 127-144.
Rebetzke, G.J., Rattey, A.R., Bovill, W.D., Richards, R.A., Brooks, B.J., Ellis, M. and Palta, J. 2022. Agronomic assessment of the durum Rht18 dwarfing gene in bread wheat. Crop Pasture Sci. 73: 325-336.
Rebetzke, G.J., Richards, R.A., Fettell, N.A., Long, M., Condon, A.G., Forrester, R.I. and Botwright, T.L. 2007. Genotypic increases in coleoptile length improves stand establishment, vigour and grain yield of deep-sown wheat. Field Crops Res. 100: 10-23.
Shah, A.N., Tanveer, M., Rehman, A.U., Anjum, S.A., Iqbal, J. and Ahmad, R. 2017. Lodging stress in cereal—effects and management: an overview. Environ Sci Pollut Res. 24: 5222-5237.
Singh, G.P., Prabhu, K.V., Singh, P.K., Singh, A.M., Jain, N., Ramya, P., Sharma, J.B., Kumar, J., Siwasami, M., Prasad, S.S. and Mishra, A.N. 2015. HD 3086: A new wheat variety for irrigated, timely sown condition of North Western Plains Zone of India. Journal of Cereal Research. 6: 160-165
Tang, T., Botwright Acuña, T., Spielmeyer, W. and Richards, R.A. 2021. Effect of gibberellin-sensitive Rht18 and gibberellin-insensitive Rht-D1b dwarfing genes on vegetative and reproductive growth in bread wheat. J Exp Bot. 72: 445-458.
Tekdal, S. and Kendal, E. 2018. AMMI model to assess durum wheat genotypes in multi-environment trials. J Agric Sci Technol. 20:153-166.
Tetland, S. 2020. Agronomic, quality, and genetic analysis of the Rht18 semi-dwarfing gene in a Canadian Western Red Spring Wheat (Triticum aestivum L.) background (Doctoral dissertation, University of Saskatchewan).
Trethowan, R.M., Mahmood, T., Ali, Z., Oldach, K. and Garcia, A.G. 2012. Breeding wheat cultivars better adapted to conservation agriculture. Field Crops Res. 132: 76-83.
Van Berloo, R. 1999. Computer note. GGT: software for the display of graphical genotypes. J Hered. 90: 328-329.
Van De Velde, K.,Thomas, S.G., Heyse, F., Kaspar, R., Van Der Straeten, D. and Rohde, A. 2021. N-terminal truncated RHT-1 proteins generated by translational reinitiation cause semi-dwarfing of wheat Green Revolution alleles. Mol Plant. 14: 679-687.
Vikhe, P., Venkatesan, S., Chavan, A., Tamhankar, S. and Patil, R. 2019. Mapping of dwarfing gene Rht14 in durum wheat and its effect on seedling vigor, internode length and plant height. Crop J. 7: 187-197.
Vikhe, P., Patil, R., Chavan, A., Oak, M. and amhankar, S. 2017. Mapping gibberellin-sensitive dwarfing locus Rht18 in durum wheat and development of SSR and SNP markers for selection in breeding. Mol Breed. 37: 1-10.
Vinod, K.K., Gopala Krishnan, S., Senapati, M. and Singh, A.K. 2022. Breeding field crops: history, current status and introspections. Fundamentals of Field Crop Breed. 1-38.
Wang, Y., Du, Y., Yang, Z., Chen, L., Condon, A.G. and Hu, Y.G. 2015. Comparing the effects of GA-responsive dwarfing genes Rht13 and Rht8 on plant height and some agronomic traits in common wheat. Field Crops Res. 179: 35-43.
Wilhelm, E.P., Boulton, M.I., Barber, T.E., Greenland, A.J. and Powell, W. 2013. Genotype analysis of the wheat semidwarf R ht‐B 1b and R ht‐D 1b ancestral lineage. Plant Breed. 132: 539-545.
Wu, J., Kong, X., Wan, J., Liu, X., Zhang, X., Guo, X., Zhou, R., Zhao, G., Jing, R., Fu, X. and Jia, J. 2011. Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1. Plant Physiol. 157: 2120-2130.
Xu, D., Bian, Y., Luo, X., Jia, C., Hao, Q., Tian, X., Cao, Q., Chen, W., Ma, W., Ni, Z. and Fu, X. 2023. Dissecting pleiotropic functions of the wheat Green Revolution gene Rht-B1b in plant morphogenesis and yield formation. Development. 150.
Yadav, R., Gaikwad, K.B. and Bhattacharyya, R. 2017. Breeding wheat for yield maximization under conservation agriculture. Indian J Genet Plant Breed. 77: 185-198.
Yadav, R., Gaikwad, K., Bhattacharyya, R., Bainsla, N.K., Kumar, M. and Yadav, S.S. 2018. Breeding new generation genotypes for conservation agriculture in maize-wheat cropping systems under climate change. Food Secur Clim Change. 189-228.
Yadav, R., Gupta, S., Gaikwad, K.B., Bainsla, N.K., Kumar, M., Babu, P., Ansari, R., Dhar, N., Dharmateja, P. and Prasad, R. 2021. Genetic gain in yield and associated changes in agronomic traits in wheat cultivars developed between 1900 and 2016 for irrigated ecosystems of northwestern plain zone of India. Front Plant Sci. 12: 719394.
Yan, W. 2015. Mega‐environment analysis and test location evaluation based on unbalanced multiyear data. Crop Sci. 55: 113-122.
Yan, W. and Tinker, N.A. 2006. Biplot analysis of multi-environment trial data: Principles and applications. Can J Plant Sci. 86: 623-645.
Yang, Z., Zheng, J., Liu, C., Wang, Y., Condon, A.G., Chen, Y. and Hu, Y.G. 2015. Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat. Field Crops Res. 183: 92-101.
Yu, G., Hatta, A., Periyannan, S., Lagudah, E. and Wulff, B.B. 2017. Isolation of wheat genomic DNA for gene mapping and cloning. Wheat Rust Dis: Methods Prot. 207-213.