Development of microsatellite markers in finger millet

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S. Mohan Kumar
J. N. Madhura
M. S. Sheshashayee
Shailaja Hittalamani
M. Udayakumar


In the present study, for the first time an attempt was made to isolate microsatellites in finger millet using pre-cloning enrichment (selective hybridization) strategy which resulted in isolation of very good number of micro satellites (323).While developing the microsatellite-enriched library for finger millet, more than 2200 colonies were screened, and 1021 recombinant clones ranging from 500-1000bp were selected for further sequencing. Sequence analysis revealed that only 645 clones (63%) contained microsatellite motif regions. Monorepeats C and T were abundant, whereas, AG and CT class of dinucleotide repeats accounted about 21% and 30% respectively. Apart from the abundant, mono, di-nucleotide and tri repeats, about 58 tetranucleotide and 62 penta repeats were also isolated.

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Kumar, S. M., Madhura, J. N., Sheshashayee, M. S., Hittalamani, S., & Udayakumar, M. (2021). Development of microsatellite markers in finger millet. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 81(03), 462–465.
Research Article