Indian Society of Genetics & Plant Breeding

Variation in phenol colour reaction in grains of rice (Oryza sativa L.) varieties

Published on

Phenol colour reaction on grains and glumes is used for
characterization of rice varieties for distinctiveness,
uniformity and stability (DUS) testing in plant variety
protection. According to DUS test guidelines, rice varieties
are classified into two groups based on presence and
absence of phenol colour reaction on the grains. However,
rice grain changes to black or light brown colour in different
frequencies in seed lots of varieties. An investigation was
conducted to differentiate 20 rice varieties based on
variations in phenol colour reaction. Studies were also
conducted to show intra-panicle and inter-plant and panicle
variation in paddy seeds based on the variation in phenol
colour response during different seed growth and maturity
stages. The results showed a negligible existence of interand intra-plant/panicle variation in grain in rice varieties
due to phenol colour reaction. The phenol colour reaction
starts at the post fertilization stage of the spikelet and is
maintained in the matured grains. Instead of two groups
the phenol colour test in the existing DUS test guidelines a
higher number (five) of groups like black, dark brown,
brown, light brown and no colour is recommended as a
grouping characteristic in DUS testing of rice varieties for
plant variety protection.

Keywords: Phenol colour test, stage of induction, variation, DUS testing, rice variety


Year: 2021
Volume: 81
Issue: 3
Article DOI: 10.31742/IJGPB.81.3.3
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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