Indian Society of Genetics & Plant Breeding

Variability in morphological characters and alkaloid profile of EMS induced mutants of Catharanthus roseus (L.) G. Don

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Seeds of Catharanthus roseus were mutagenised with ethyl
methane sulphonate. On screening induced variability ten
morphological variants (NV1-NV10) were isolated and their
morphological variability was assessed. The hierarchical
clustering analysis revealed four major groups based on
morphological attributes. Genotypes with higher total
alkaloid content in leaf were placed together in cluster-I
and higher leaf yield plant–1, root yield plant–1 and total
alkaloid content in root were placed in cluster-II. Genotypes
with lowest mean values of all mentioned traits except leaf
lamina width and petiole length were placed in cluster III.
The consistency in performance of NV-1 and NV-10 from M1
to M3 without any significant variance in their progeny
traits, showed that these were true breeding mutants.
Alkaloid profiling of these ten variants was carried out by
using recently developed DART MS technique. Marked
variations in the alkaloid profile were also noted among
them which signify the use of induced mutation in
developing desirable ‘chemocultivars’.

Keywords: C. roseus, induced mutagenesis, EMS, variant, DART MS technique


Year: 2014
Volume: 74
Issue: 3
Article DOI: 10.5958/0975-6906.2014.00854.2
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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