Indian Society of Genetics & Plant Breeding

Validation of rapid DNA extraction protocol and their effectiveness in marker assisted selection in crop plants

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In the present study, a simple and ultra quick protocol for
extraction of genomic DNA was successfully validated in
four different crops namely rice, wheat, lentil and Indian
mustard.The method is simple and involves only two steps,
it does not involve use of liquid nitrogen and other
expensive and toxic reagents, and is highly suited for
analyzing large number of samples within a short span of
time. This makes it very attractive for use in MAS even in
smaller labs where no sophisticated facilities are available.

Keywords: Crops, marker assisted selection, DNA extraction, validation


Year: 2015
Volume: 75
Issue: 1
Article DOI: 10.5958/0975-6906.2015.00015.2
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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