Validation of molecular markers linked to the stem rust resistance genes effective in India

Published on Jun 30, 2018 10:16 AM

An investigation was carried out to validate the already
known molecular markers Xgwm533, Xcfa2019 & Xcfa2123,
Sr24#12 & Sr24#50, Sr26#43 and BE518379, SCSS30.2 and
Sr39-I & Sr39-II for stem rust resistance genes Sr2, Sr22,
Sr24, Sr26, Sr31 and Sr39, respectively in wheat cultivars
Kundan and UP 2338 carrying unknown stem rust resistance
gene(s). The Sr2 and Sr22 gene specific SSR markers
amplified the gene specific or expected allele in both Sr2
andSr22 carrying and non-carrying cultivars.Sr24associated
STS markers (Sr24#12 & Sr24#50) amplified their respective
expected fragments (500 bp and 200 bp) only in known
Sr24 gene carrying cultivar, HD 2851. Two DNA markers
(Sr26#43 & BE518379) known for Sr26 gene were screened.
Marker, Sr26#43 amplified the specific fragment of 207 bp
only in known source carrying Sr26 gene,while other marker,
BE518379 functioned as null allele marker by not amplifying
the critical band in Sr26 lines. SCAR marker, SCSS30.2
specific to Sr31 gene amplified the gene specific fragment
in wheat cultivar UP 2338 confirming the presence of Sr31.
Out of 14 entries tested for Sr39 gene including Kundan
and UP 2338, the expected PCR product was not amplified
in any of the entries indicating absence of Sr39 gene.

Keywords: Wheat, stem rust resistance genes, molecular markers, validation

Info

Year: 2013
Volume: 73
Issue: 3
Article DOI: 10.5958/j.0975-6906.73.3.046
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

N. R. Nagaraja, Anupam Singh, J. K. Pallavi, J. B. Sharma, G. P. Singh, Neelu Jain and K. V. Prabhu info_circle

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