Indian Society of Genetics & Plant Breeding

Validation of molecular markers linked to low glucosinolate QTLs for marker assisted selection in Indian mustard (Brassica juncea L. Czern & Coss)

Published on

Six earlier reported markers closely linked to low
glucosinolate QTLs of Brassica juncea, spread across ‘A’
genome (A2, A3 and A9) were validated in a recombinant
inbred line (RIL) population of a cross between Pusa
Mustard-21 (low erucic acid) and EC-597325 (double low)
genotypes, to utilize them in marker-assisted selection
(MAS). Of them, four markers viz., GER 1 amplified alleles
of 650 bp and of 950 bp, GER 5 amplified 310 bp and 350 bp,
At5gAJ67 amplified 500 bp and 450 bp and Myb28 amplified
alleles of size 900 bp and 920 bp in EC597325 and Pusa
Mustard-21, respectively and therefore differentiated low
and high glucosinolate parents. These four polymorphic
markers were then used to genotype the phenotyped RIL
population consisting 608 plants. Marker-trait association
was tested for goodness of fit using χ2 test. Of the four
markers, GER1 and GER5 showed higher phenotypic
variance (R2 value) compared to the others, indicating their
significance in determination of glucosinolates and
prospects for use in MAS for development of Indian mustard
genotypes with low glucosinolates content.

Keywords: Indian mustard, oil quality, molecular markers, glucosimolates, QTLs


Year: 2016
Volume: 76
Issue: 1
Article DOI: 10.5958/0975-6906.2016.00009.2
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Devendra K. Yadava info_circle
Naveen Singh info_circle
Sujata Vasudev info_circle
Navinder Saini info_circle
Vignesh Muthusamy info_circle
Kumble Vinod Prabhu info_circle

Download PDF