Indian Society of Genetics & Plant Breeding

Validation of foreground and background SSR markers for introgression of QTL governing leaf glossiness into a sorghum variety - NTJ 2

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Leaf glossiness and trichomes on leaf surface, two
important component traits governing resistance to shoot
fly in sorghum is controlled by Quantitative trait loci (QTLs)
located on linkage group SBI-05. Foreground and
background markers were validated for transfer of the
QTLs to NTJ2, a popular variety of sorghum. For
foreground selection, five SSR markers, Xtxp268,
Xisep1111, Xtxp065, Xtxp303, and Xiabt440 in the region
of the QTL were identified from 17 tested markers. From a
total of 167 SSR markers spanning the entire genome, 35
polymorphic SSR markers, distributed on all the 10
chromosomes were identified for background selection.
The F1, BC1F1 and F2 generation plants were screened
using foreground markers. Based on the results obtained,
the hybridity of the F1’s was confirmed and markerhomozygote individuals in F2 generation were identified
for subsequent backcrossing.

Keywords: Sorghum, shoot fly, QTL, leaf glossiness, foreground selection, background selection, introgression


Year: 2012
Volume: 72
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


P. Janila info_circle
G. Anuradha and A. Shivashanka info_circle

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