Indian Society of Genetics & Plant Breeding

Validation of chickpea-STMS markers and DNA fingerprinting in lentil (Lens culinaris subsp. culinaris) cultivars of India

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A set of 31 lentil genotypes having indigenous and exotic
origin were screened using 42 STMS markers derived from
chickpea genome. Out of the 42 STMS primers tested, all
of them gave amplified products in lentil. Among them 36
(85.7%) primers were found to be polymorphic among the
lentil genotypes. At genetic distance of less than 0.29, all
the 31 genotypes could be grouped into five clusters where
cluster IV contained 19 genotypes, including the exotic
genotype Precoz. Many primers gave genotype-specific
amplified products which in combination(s) could be used
for DNA fingerprinting of lentil genotypes. A set of 16 STMS
markers have been identified which could differentiate all
the lentil cultivars of India and, along with the
morphological data can be used for identification and
confirmation of the purity of the lentil cultivars.

Keywords: Lentil, fingerprinting, diversity, SSR, STMS


Year: 2008
Volume: 68
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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