Indian Society of Genetics & Plant Breeding
Molecular markers for the leaf rust resistance genes Lr9,
Lr19, Lr24, rye specific chromatin (1 BL.1 RS translocation,
Lr26), and Lr28 were validated in the parental lines of
wheat (Triticum aestivum L.). The markers for Lr9, Lr19
and Lr26 were able to discriminate the lines with specific
genes/chromatin from the lines that did not carry these
genes. The Lr24 marker showed polymorphism between
positive and negative controls but in white grained
genotypes the amplified band was faint and inconsistent
as compared to red seeded lines. The marker used for
detecting Lr28 was not polymorphic among the tested
carrier and non-carrier lines. The markers of Lr19 and
Lr24 were utilized for authenticating the presence of
specific genes in the advanced breeding lines whereas
rye-chromatin specific marker was used for marker assisted
selection of Lr26. Genetic stocks with combined resistance
genes Lr19 + Lr26 + 5r25 + 5r31 + Yr9 + Yr27 and Lr24
+ Lr26 + 5r24 + 5r31 + Yr9 + Yr27 were developed in
the background of PBW 343 with the aid of pedigree
information, host-pathogen interaction and molecular
marker.
Keywords: Wheat, leaf rust resistance, molecular markers, gene pyramiding, marker validation
Year: 2007
Volume: 67
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
D. Datta, M. Prashar and S. C. Bhardwaj info_circle
Download PDF