Indian Society of Genetics & Plant Breeding

Tri-parental protoplast fusion of Brassica species to produce somatic hybrids with high genetic and phenotypic variability

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Plating efficiencies and the plant regeneration frequencies
of bi-parental fusion of Brassica campestris pekinensis with
Ogura cytoplasmic male sterility (CMS), B. oleracea L. var.
italica with Ogura CMS, and Brassica juncea var. crispifolia
were compared for illustrating the advantages of tri-parental
somatic hybridization. The results showed that in the triparental
fusion combination, 73 plants regenerated from
712 calli and the plant regeneration frequency was 10.3%,
three or four times that obtained with the 2 fusion
combinations. These hybrids were classified into 12 types
based on morphology, and most showed intermediate
characteristics between 2 or more of the parental species.
The somatic hybiridity were confirmed by flow cytometry,
genomic in situ hybridization (GISH) and PCR analysis,
indicating that these regenerated plants were all true
hybrids. Most of the progenies with normal pollen showed
varied number of seed set after backcrossing with B. juncea.
The high variability in the hybrids obtained illustrated that
somatic hybridization may be useful in broadening existing
Brassica gene pools and obtaining material for breeding.

Keywords: Brassica, tri-parental protoplast fusion, genomic in situ hybridization, flow cytometry, morphology

Info

Year: 2015
Volume: 75
Issue: 4
Article DOI: 10.5958/0975-6906.2015.00079.6
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Qin Zheng info_circle

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