Indian Society of Genetics & Plant Breeding

Transferability of SSR markers across twelve species of forage legumes for germplasm characterization and evaluation

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Simple sequence repeat (SSR) or microsatellite marker is
the most preferred and reliable molecular marker system.
However, development and use of such markers for
evolution and diversity assessment is limited to few
tropical forage legumes. In the present investigation, 43
SSR markers of Medicago truncatula and Trifolium repens
from existing data bases were used to assess their
transferability in 24 accessions of 12 tropical legume
species of forage importance. Cross-genus amplification
of M. truncatula based SSR was 32.08% whereas only
23.97% transferability of T. repens markers was observed.
Seventy six polymorphic amplicons were identified could
be used as DNA markers. Identified markers were
demonstrated in characterization of 24 accessions of
Desmanthus, thus indicated their suitability. The transfer
of SSR markers across species or genus can be a very
efficient approach for DNA markers development,
especially for less explored legumes of tropical forage

Keywords: Forage legume, genetic resources, SSR, transferability


Year: 2008
Volume: 68
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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