Indian Society of Genetics & Plant Breeding

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Transfer of leaf rust and stripe rust resistance genes Lr19 and Yr15 in to a susceptible wheat cultivar HS295

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The present study was conducted to transfer multiple rust
resistance in a popular but rust susceptible wheat cultivar
HS295. Selected derivatives WBM3632 and WBM3635 have
been developed from a cross, HS295*2/FLW20//HS295*2/
FLW13 using bulk-pedigree method of breeding. Advance
line WBM3697 selected from a breeding line WBM3532 was
named as HS661. This line was evaluated for seedling
resistance to a wide array of rust pathotypes and found to
possess resistance to all the three rusts. HS661 was also
tested under field conditions and showed adult plant
resistance to leaf rust (AC1=0.6), stem rust (ACI=2.7) and
strpe rust (AC1=3.8). Among 34 F3 lines, 28 were tested
positive for SSR marker Xwmc221 indicating the presence
of Lr19/Sr25. Out of 14 selected F4 lines from F3, nine were
homozygous positive for Lr19/Sr25. The advanced breeding
lines viz., WBM3632 (WBM3697) and WBM3635 were also
positive for Lr19/Sr25 with SCAR marker SCS265512. SSR
marker Xgwm1 producing 215 bp band in Avst-15, FLW13
and HS661 confirmed the presence of Yr15 . Agronomically,
HS661 was comparable with recipient variety HS295 and
superior to a standard check HS490 under late sown
restricted irrigation production conditions of NHZ. HS661
may serve as a potential donor for creating new usable
variability against all the three rusts.
 

Keywords: Wheat, SSR marker, Lr19/Sr25, Yr15

Info

Year: 2019
Volume: 79
Issue: 3
Article DOI: 10.31742/IJGPB.79.3.12
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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