Indian Society of Genetics & Plant Breeding

Transfer of cytoplasmic male sterility from alloplasmic Brassica juncea and B. napus to cauliflower (B. oleracea var. botrytis) through interspecific hybridization and embryo culture

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Cytoplasmic male sterile (CMS) lines of Brassica juncea
and B. napus with the mitochondrial genome of Moricandia
arvensisand Erucastrum canariense, respectively, were used
to transfer CMS to cauliflower (B. oleracea). Embryo culture
was essential to recover these interspecific hybrids. Success
of embryo rescue was dependent on the post-pollination
age of the ovary; ovaries excised at 9- and 14- days-afterpollination gave the best response in B. napus x B. oleracea
and B. juncea x B. oleracea, respectively. Embryo rescue
was also necessary in BC1 and BC2 to obtain progenies.
Recovery of the recurrent parent phenotype was faster in
B. napus x B. oleracea than B. juncea x B. oleracea. BC3
generation plants of B. napus x B. oleracea showed good
curd formation and complete male sterility and nine
bivalents at meiosis whereas those of B. juncea x B. oleracea
were male sterile but still had genetic elements of B. juncea.

Keywords: Brassica oleracea var. botrytis, cauliflower, CMS, Erucastrum canariense, Moricandia arvensis


Year: 2013
Volume: 73
Issue: 2
Article DOI: 10.5958/j.0975-6906.73.2.030
Print ISSN: 0019-5200
Online ISSN: 0975-6906


H. S. Balyan info_circle
S. R. Bhat info_circle

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