Indian Society of Genetics & Plant Breeding

Techniques to produce hybrid between Cicer arietinum L. x C. pinnatifidum Jaub.

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Androgenesis has not been reported in chickpea (Cicer
arietinum L.). Interspecific hybridization between C.
arietinum and C. pinnatifidum was possible by hormoneaided pollinations, embryo rescue and tissue culture
techniques to save aborting hybrid embryos. Since the
hybrids did not have a good root system, hybrid shoots
were grafted to cultivated chickpea stocks. By this method
it was possible to transfer hybrids to soil. Hybrid plants
were fragile and were maintained in a growth room. Hybrid
plants flowered only when the cytokinin zeatin (1 mg/l)
was added to the sterilized tap water used to water the
plants. Flowers were pale violet and cleistogamous. All of
the components of the flower were present, although the
anthers did not dehise. Anthers squashed in acetocarmine
revealed from 4-10 divisions in many of the microspores.
Induction of androgenesis is believed to be due to wide
hybridization between C. arietinum and C. pinnatifidum.

Keywords: Androgenesis, Cicer arietinum, Cicer pinnatifidum, cleft graft, interspecific hybridization


Year: 2008
Volume: 68
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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