Indian Society of Genetics & Plant Breeding

Tagging of Terminal Flowering Locus (TFL) homologue responsible for growth habit in Indian bean [Lablab purpureus (L.) Sweet]

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The growth habit and photoperiod responsive flowering
might have shaped domestication and evolution of Indian
bean. Determinate growth habit enables alternative cropping
systems and diversification. In the present study, a cross
was made between determinate variety GNIB-21 and
indeterminate genotype GPKH-120 to identify gene
responsible for growth habit. The hybrids had indeterminate
nature endowed from male parent, while growth habit
exhibited 3: 1 segregation in F
2 population suggesting
monogenic control of the trait. Bulk segregant analysis
indicated association of
TFL (Terminal flowering locus)
specific amplicon with growth habit. This amplicon was
present in parent GPKH-120, F
1 hybrid, germplasm
accessions and F
2 individuals with indeterminate growth
habit, while it was absent in determinate parent GNIB-21
and determinate F
2 progenies revealing involvement of TFL
locus in governing growth habit. Homology search revealed
89%, 78% and 89% identity with common bean, soybean
and cowpea loci,
PvTFL1Y, GmDt1-h and VuTFL1,
respectively. ClustalW analysis indicated that the partial
sequence of
TFL amplified belongs to first intron of gene.
This result will enable identification and characterization
of gene responsible for growth habit eventually facilitating
manipulation of plant architecture in Indian bean.

Keywords: Terminal flowering locus, growth habit, bulk segregant analysis, homologue, multiple sequence alignment

Info

Year: 2021
Volume: 81
Issue: 3
Article DOI: 10.31742/IJGPB.81.3.12
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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