Indian Society of Genetics & Plant Breeding

Successful deployment of marker assisted selection (MAS) for inbred and hybrid development in long-day onion (Allium cepa L.)

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In the present investigation, male sterile and maintainer
lines were identified using molecular markers in three long
day onion populations. Molecular markers, 5’cob and
orfA501 were able to distinguish effectively normal (N) and
sterile (S) cytoplasm in all the three populations. The
observed frequency of S cytoplasm in VL Piaz 67 (100%),VL
Piaz3 (86.4%) and KR1 (90%) was higher than N cytoplasm.
Out of the two PCR markers viz., OPT and PsaO used to
determine the nuclear fertility restorer locus (Ms locus),
OPT was found better than PsaO. Increased frequency of
dominant homozygous alleles (87.4%) followed by
heterozygous alleles (8.6%) and homozygous recessive
alleles (4.0%) was observed. Fertility/sterility of plants were
validated by acetocarmine staining of the pollens and
correlated with observations made using molecular markers.
Out of the three populations studied, only 12 plants (2.15%)
happened to be completely male sterile in VL Piaz-3 and
KR1 population, whereas there was no male sterile plant
observed among the population in VL Piaz 67. Selfing and
test crossing of plants having normal (N) cytoplasm led to
the development and identification of maintainer lines in
VL Piaz3 and KR1 population. This is the first example of
deploying DNA markers for identification and purification
of male sterility and hybrid development in long day
onion in Indian population.

Keywords: Onion, Allium cepa L., cytoplasmic male sterility, molecular markers, Ms locus


Year: 2015
Volume: 75
Issue: 1
Article DOI: 10.5958/0975-6906.2015.00012.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Nirmal K. Hedau, Shailendra Yadav, Jagadish C. Bha info_circle
Anil Khar info_circle

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