Indian Society of Genetics & Plant Breeding

STUDIES ON CALLUS GROWTH AND DIFFERENTIATION IN SAFFLOWER

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Tissue culture studies were carried out to standardise hormonal requirements and to
determine the best source of explant (cotyledonary leaf, hypocotyl and root) for callus
induction, growth and differentiation in two safflower genotypes MS-I05 (Male sterile)
line and Manjira. Murashige-Skoog medium supplemented with 5.0 mg r 1 naphthalene
acetic acid (NAA) and 0.25 mg 1-1 6 benzyl aminopurine (BAP) induced maximum callus
(65.40/.) irrespective of genotype and explant. Cotyledonary leaf was found to be efficient
for callus induction, growth and differentiation. MS medium supplemented with 2.0 mg
1-1 NAA was significantly superior to other hormonal combinations in terms for
differentiation. Frequency of direct rhizogenesis and caulogenesis was maximum from
bothcotyledonaryleafand hypocotyl explants cultured onMS medium supplementedwith
5.0 mg 1-1 NAA and 0.1 mg 1- 1NAA + 0.25 mg 1- 1 BAP, respectively.
 

Keywords: Safflower, callus, differentiation, caulogenesis, rhizogenesis.

Info

Year: 1996
Volume: 56
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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