Indian Society of Genetics & Plant Breeding

Status and prospects of gene editing and transgenic in fishes

Published on

Recent advances in high throughout DNA sequencing
technologies have revolutionized for better understanding
of structure-functional relationships of genes in identifying
trait-associated transcriptomes and their regulated gene
expressions. Subsequent breakthroughs in gene editing
technologies such as zinc finger nucleases, transcriptional
activator-like effect or nucleases (TALEN) and CRISPR
(clustered regularly interspaced short palindromic repeats)
determined chromosomal loci so as to understand gene
functions in vivo. Such editing technologies are now being
implemented in many laboratories due to an affordable
cost and easiness of techniques. Targeted gene delivery
and disruptions are now not only restricted to standard
cell lines or stem cells, but also primary cell lines and nonmodel agriculturally important species. Progress and
implications of gene integration and disruptions in food
fishes like salmon, carps, etc. will be highlighted. The
positive impacts on myostatin gene (negative growth
hormone regulator) disruption mediated muscular growth
have been documented. Transposon mediated gene
integration technologies for value-additions to small
indigenous aquarium fishes by expressing attractive
fluorescent color genes could be the future of rainbow
revolution. Issues linked with further-tuning with regards
to improved efficacy and specificity, while reducing offtarget effects of gene editing tools will be addressed. There
are health and environmental concerns with genetically
modified organisms (GMOs). CRISPR/Cas9 mediated
editing generates indels and hence supposed to be free
from transgene-nontoxic and non-allergen. Scientific
progress regarding to generate genetically modified carps;
those could well be cultivated in a confinement and at the
same time economically profitable; will be highlighted.
Emphasis should be given for transfer these technologies
from the laboratory to land for the development of a
consumer-friendly sustainable farming system

Keywords: Genetically modified organisms (GMOs), gene editing, CRISPR/Cas9, transgenic fishes.


Year: 2019
Volume: 79
Issue: 1, Supplement
Article DOI: 10.31742/IJGPB.79S.1.21
Print ISSN: 0019-5200
Online ISSN: 0975-6906



Download PDF