Indian Society of Genetics & Plant Breeding

Site specific mutagenesis in nodulation genes of Mesorhizobium ciceri strain Me 18-7 using in vitro transposition in a heterologous cloned DNA

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Mesorhizobium eieeri strain MC18-7, nodulating chickpea
was used to generate nod-mutants to study primary
nodulation responses. Transposon insertion was carried
out in pRmSL-26 using in vitro transposition by EZ:: TN
<KAN 2> insertion system. Transposon insertion sites in
nod ABC and nod 0 genes in pRmSL-26 were used for
marker exchanging in M. eieeri strain MC 18-7. In total
three mutants, one nod ABC- mutant and two nod 0-
mutants were obtained. The common nod ABC- mutant
showed complete lack of root hair infection/deformation,
while nod 0- mutants showed delayed/reduced primary
modulation responses.

Keywords: Chickpea, Mesorhizobium ciceri, nodulation genes, in vitro transposition, marker exchanging


Year: 2003
Volume: 63
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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