Indian Society of Genetics & Plant Breeding

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Simple DNA extraction method for SSR-PCR analysis from different tissues of Tapiscia sinensis, an endangered plant species

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A simple and rapid method to process different tissues of
Tapiscia sinensis for effective plant DNA extraction were
established in the study. DNA quality and yield obtained
from bark, leaves and seeds were evaluated by Eppendorf
BioPhotometer and SSR-PCR analysis. The results indicated
that extracted genomic DNA based on the established
method was sufficient for SSR-PCR profiling of different
tree tissues. Hence, the sample treatment and extraction
method can be used for SSR-PCR applications in tree
genetic and molecular analysis research.

Keywords: Tapiscia sinensis, sample treatment, DNA extraction, SSR-PCR

Info

Year: 2015
Volume: 75
Issue: 3
Article DOI: 10.5958/0975-6906.2015.00063.2
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Rong-Shan Yan, Ya-Nan Xu, Da-Wei Zou, Peng Zhao an info_circle

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