Selection of reliable reference genes for gene expression studies by quantitative real-time PCR in Anoectochilus roxburghii

Published on May 25, 2018 02:24 AM

RT-qPCR is a sensitive, efficient and reliable method for
gene expression studies and reference gene expression
stability was essential for RT-qPCR. Since previous studies
showed that no reference gene could exhibit changeless
expression pattern in all experiments, this study determined
the gene expression stability of 11 reference genes across
various treatments in Anoectochilus roxburghii. The results
indicated that expression stability of genes varied
considerably under different treatments. The genes, GAPDH,
EF1a and ACT1 emerged as the most reliable reference
genes.

Keywords: Anoectochilus roxburghii, geNorm, NormFinder, reference gene

Info

Year: 2017
Volume: 77
Issue: 3
Article DOI: 10.5958/0975-6906.2017.00060.8
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Yanan Zhang info_circle
Boyun Yang info_circle
Liping Luo info_circle
Dongjin Xiong info_circle
Xueyong Huang info_circle
Huolin Luo info_circle

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