Indian Society of Genetics & Plant Breeding

Selection of reliable reference genes for gene expression studies by quantitative real-time PCR in Anoectochilus roxburghii

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RT-qPCR is a sensitive, efficient and reliable method for
gene expression studies and reference gene expression
stability was essential for RT-qPCR. Since previous studies
showed that no reference gene could exhibit changeless
expression pattern in all experiments, this study determined
the gene expression stability of 11 reference genes across
various treatments in Anoectochilus roxburghii. The results
indicated that expression stability of genes varied
considerably under different treatments. The genes, GAPDH,
EF1a and ACT1 emerged as the most reliable reference

Keywords: Anoectochilus roxburghii, geNorm, NormFinder, reference gene


Year: 2017
Volume: 77
Issue: 3
Article DOI: 10.5958/0975-6906.2017.00060.8
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Boyun Yang info_circle
Liping Luo info_circle
Dongjin Xiong info_circle
Xueyong Huang info_circle
Huolin Luo info_circle

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