Indian Society of Genetics & Plant Breeding

Selection of optimal q RT-PCR reference genes for gene expression studies in foxtail millet

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Appropriate reference gene is very important for gene
expression analysis by the quantitative RT-PCR (qRT-PCR),
but no systematics research have been carried out in foxtail
millet. In this study, the mRNA expression were investigated
by qRT-PCR for six candidate reference genes, 25S rRNA,
18S rRNA, 17S rRNA, GAPDH, ACTB and UBQ under three
different experimental conditions including different stages
of leaf rust, five plant organs and seven different foxtail
millet cultivars. The results obtained with GeNorm,
NormFinder and BestKeeper software tools showed stable
and uniform expression of 25S rRNA among different
cultivars, 18S rRNA under the different conditions of leaf
rust infection and 17S rRNA in different foxtail millet organs
The list of reference genes confirmed in present study will
be useful for genes expression analysis in foxtail millet
under different conditions.

Keywords: Foxtail millet, reference genes, geNorm, normFinder, bestKeeper


Year: 2015
Volume: 75
Issue: 2
Article DOI: 10.5958/0975-6906.2015.00034.6
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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