Indian Society of Genetics & Plant Breeding

Screening of wheat varieties for use as donors for production of near isogenic lines with different seed protein alleles

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Sequential extraction of water-soluble albumins,
salt-soluble globulins (triticin), alcohol-soluble gliadins and
acid-soluble glutenins, the four major classes of wheat
seed storage proteins, was done from 31 wheat cultivars.
SOS-PAGE of the protein fractions showed high variation
in the banding pattern. Varieties with different alleles in
comparison to the recurrent parent HO 2329 were chosen
for all the four fractions of seed storage proteins. A
total of 12 cultivars were finally chosen to make NILs for
forty different seed storage protein alleles in HO 2329.

Keywords: Wheat, seed storage proteins, allelic variation, SDS-PAGE. NILs


Year: 2003
Volume: 63
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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