Indian Society of Genetics & Plant Breeding

Screening of soybean [Glycine max (L.) Merrill] genotypes for somatic embryogenesis and plant regeneration potential

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Twenty two soybean genotypes were evaluated for the
capacity of embryo initiation, differentiation, maturation
and plantlet regeneration using standardized protocols.
Among the genotypes, Bragg was to have high response
to induction with 91.11% and average 38.40 embryos/callus
mass), proliferation (89.47%) and differentiation (78.95%)
however, SL 688 gave the highest maturation frequency
(91.57%). Highest somatic embryogenesis germination was
recorded in SL 525 (85.67%) whereas, highest survival was
observed in JS 335 (82.22%). Genotype Pusa 37 gave the
poor response. Fifteen genotypes which were identified as
responsive were Bragg, Bragg-Palampur, Pusa 5, Pusa 12,
Pusa 14, Pusa 16, Pusa 24, Pusa 40, Pusa 9814, SL 688, SL
525, SL 979, SL 958, DS 2706 and DS 2708. The study thus
open up vistas for efficient transgenic plant development
in soybean.

Keywords: Glycine max L., immature cotyledon, somatic embryogenesis, regeneration.


Year: 2017
Volume: 77
Issue: 3
Article DOI: 10.5958/0975-6906.2017.00052.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906


S. K. Lal info_circle
S. K. Singh info_circle
A. Talukdar info_circle
Vinod info_circle

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