Indian Society of Genetics & Plant Breeding

RNA-Seq analysis of two elite lines of Withania somnifera to evaluate potential of heterosis

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Metabolite targeted breeding is one of the prime need in
Ashwagandha (Withania somnifera) to meet its fast
growing demand at domestic and international level owing
to its vast spectral pharmaceutical potential.Till date only
scanty breeding efforts exists majority being introductions
or selections from the wilds/landraces through which
active bio constituent increase is limited. Hybridization
based heterotic positive QTL alleles can be introgressed
in a fine quality good agronomic genetic base using marker
assisted selection. Investigations under this study
generated a large resource of Withania specific genomic
microsatellite markers and SNP through RNA seq. Using
an RNA-seq approach, the expression of 101245 unigenes
were quantified in parents .The expression levels of 30%
of transcripts differed between parents, a majority of which
had more than 1.5 fold-changes.RNA seq will help to
decipher the Withanolide biosynthesis pathway and SNP
responsible for functional polymorphism in pathway genes
and hence predicting the potential of heterosis

Keywords: Withania somnifera, heterosis, RNA- seq, withanolides, SNP


Year: 2014
Volume: 74
Issue: Supplementry Issue
Article DOI: 10.5958/0975-6906.2014.00899.2
Print ISSN: 0019-5200
Online ISSN: 0975-6906


K. Shanker info_circle
A. K. Gupta and Tripta Jhang info_circle

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