Indian Society of Genetics & Plant Breeding

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RNA editing in CMS wheat: Influence of nuclear background leads to differential editing on orf 256

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RNA editing and cytoplasmic male sterility are two
important and correlated phenomenons in mitochondria
of higher plants. We investigated the occurrence of RNA
editing in orf 256, which is implicated in causing male
sterility in wheat in two different CMS lines carrying
different cytoplasms along with their fertility restored lines.
This study provides the first preliminary report of RNA
editing in orf 256 with greater frequency observed in a
fertility-restored line compared to male sterile line carrying
the T.timopheevi cytoplasm. In order to investigate whether
a similar process occurs in a line carrying the T.araraticum
cytoplasm, it was observed that the male sterile line
showed a complete lack of editing in orf 256 whereas in
the restored hybrids there was an increase in frequency
of editing. Editing sites were also found conserved in both
the hybrids at 381 position of C residue indicating the non
random nature of editing especially under different nuclear
backgrounds. Thus RNA editing might be involved in either
causing male sterility or restoration of fertility in these
two systems suggesting that a common mechanism may
exist in these two different cytoplasms.
 

Keywords: Cytoplasmic male sterility, wheat, RNA editing, codon, orf 256

Info

Year: 2008
Volume: 68
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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A. Singh, K. Gaikwad, N. K. Singh info_circle
Vinod,S. M. S. Tomar info_circle

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