Indian Society of Genetics & Plant Breeding

Response of wheat crosses involving salt tolerant genotypes to anther culture

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Anthers of seven wheat (Triticum aestivum L) genotypes
and their eight F1 hybrids involving Kharchia375 and
Kharchia65, salt tolerant cultivars, as female parents were
cultured on four modified media, both solid and liquid.
The per cent anthers forming calli varied between 0 and
9.69 depending upon the genotype, cross and culture
medium. Calli yielded both green (upto 5.6%) and albino
plantlets (8.5 to 48.9%) on transfer to solidified agar 190-2
regeneration medium. Crosses differed significantly and
the best cross with respect to callusing and green plantlet
regeneration was Kharchia375 x HD2009. In general
responses of different crosses were significantly better
in liquid media and the most effective medium overall
was P
2 followed by P4, BAC1 and Heh. Higher green
plantlet regeneration abilities from anthers of F1 hybrids
as compared to the parents suggest their use to increase
green plant yield. Plantlets thus produced from crosses
after diploidization were transferred to pots. Ninety-seven
fertile spikes obtained from crosses showed a wide
variation for spike length, spikelet number per spike and
grain number per spike. The study may be helpful for
the development of salt tolerant doubled haploid lines of

Keywords: Wheat, anther culture, callus induction, green plantlets


Year: 2002
Volume: 62
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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