Indian Society of Genetics & Plant Breeding

Recovery of fertile transgenic plants via Agrobacterium tumefaciensmediated transformation in Indian soybean (Glycine max L. Merrill) cultivar

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Transgenic plants of an Indian soybean (Glycine max L.
Merrill) cultivar JS335 were obtained using Agrobacteriummediated transformation. Half seed explants were
inoculated with A. tumefaciens strain EHA 105 harboring
pBI121 containing β-glucuronidase (GUS) reporter gene
and neomycin phosphotransferase (nptII) as selectable
marker gene. Selection of regenerated primary plants was
carried out in a medium with sub-lethal dose of kanamycin
(100-175 mg/L). Transformed plants were recovered with
a success rate of 0.91%. Stable integration and expression
of transgenes in T0 plants were confirmed by southern
blot and histochemical assay respectively. The T0 plants
were fully fertile, with no apparent phenotypic
abnormalities. Analysis of the T1 progeny and T4 plants
from four independent events showed the stable
inheritance of GUS gene

Keywords: Agrobacterium, half-seed, kanamycin, soybean, transformation


Year: 2012
Volume: 72
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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