Indian Society of Genetics & Plant Breeding


RAPD based DNA fingerprinting and analysis of genetic diversity in radiation induced mutants of cowpea [Vigna unguiculata (L.) Walp.]

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Thirteen radiation-induced mutants of cowpea [Vigna
unguiculata (L.) Walp.] cv. V-130 showing distinct
morphological differences, besides the parental line, were
screened for random amplified polymorphic DNA (RAPD)
variation. Sixty-four random decamer primers used for
amplification generated a total of 495 bands, of which
230 (46.5%) were polymorphic. Mutant-specific
polymorphic markers either alone or in combination, were
detected. Eight mutants and the parent can be identified
by using specific markers, while combination of two
markers could identify three other mutants. The Jaccard's
similarity coefficient revealed considerable genetic diversity
among the mutants. The dissimilarity between the mutants
was as high as 61 per cent. The UPGMA based
dendrogram showed two clusters, as supported by
bootstrapping, with seven sub-clusters. The high range
of genetic diversity observed among the mutants affirms
the potentiality of radiation in inducing variability in
cowpea. DNA fingerprinting of the mutants will facilitate
their identification, registration and determination of seed

Keywords: Cowpea. DNA fingerprinting, genetic diversity. mutants, RAPD markers


Year: 2004
Volume: 64
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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