Indian Society of Genetics & Plant Breeding

RAPD and minisatellite markers for genetic diversity and relationship in guava varieties

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PCR based Random Amplified Polymorphic DNA (RAPD)
and Directed Amplification of Minisatellite DNA (DAMD)
markers were used to study the genetic diversity and
relatedness among 22 guava accessions comprising
commercial cultivars, breeding lines ‘and unimproved
cultivars. DNA isolated by CTAB method was used for
amplification of 96 markers by using 7 RAPD primers and
56 workers generated by 40 DAMD primers. Genetic
distance matrix based on Jaccard’s coefficient revealed
maximum distance between Purple Guava and Allahabad
Safeda (43%), whereas minimum distance was as low as
5.4% between two breeding lines HPSI-20 and HPSI-26.
Interestingly half-sib progenies CISH-G-1 to CISH-G-6 had
slightly more distance ranging from 10.8-24.0%. The
clustering revealed that most of the cultivars/accessions
originated from Indo-Gangetic plains are grouped together.
DAMD was found to suitably cluster the cultivars from
exotic origin or having exotic parentage.

Keywords: RAPD, DAMD, genetic diversity, relationship, neighbour-joining, Jaccard’s coefficient


Year: 2008
Volume: 68
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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