Indian Society of Genetics & Plant Breeding

Random amplified polymorphic DNA analysis of indigenous small and medium-grained scented rices (Oryza sativa L.) of Orissa

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Scented rices are highly preferred due to their pleasant
aroma and palatability. A large number of indigenous
aromatic rice collections are awaiting systematic study
for the assessment of genetic diversity. Random amplified
polymorphic DNA technique was used for genetic diversity
analysis among 24 rice cultivars consisting of 20 nonbasmati scented and 4 non-scented rice varieties collected
from Orissa. A total of 86 bands were amplified by 15 pre
screened polymorphic decamer primers at an average rate
of 5.73 bands/primer. The polymorphic loci recorded in
the present study clearly discriminate all the accessions.
The pair-wise genetic similarity coefficient varied from 0.52
between three pairs of rice genotypes viz., Samudrabali -
Nariyaphula, Gayatri-Nadiarasa and IR64 - Gurmatia to 0.94
between Gurmatia and Samudrabali. The UPGMA cluster
analysis revealed considerable variability among the
population of 24 cultivars and discriminated all the nonscented rices from scented rice varieties. All the 24 rice
genotypes were classified into three clusters and
separated the non-scented rice varieties from rest of the
scented rices. The group consisting of Nadiarasa and
Nariyaphula with pair wise similarity coefficient 0.75 was
separated from rest of the varieties at 60% similarity level.
No duplicate entry was detected among the 24 rice
cultivars included in the present study.

Keywords: Scented rice, RAPD, cultivar identification, genetic diversity, Oryza sativa


Year: 2008
Volume: 68
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Mohd. Arif,U. S. Singh info_circle
C. S. Kar info_circle

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