Indian Society of Genetics & Plant Breeding

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QTL mapping for relative water content trait at reproductive stage drought stress in rice

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Drought is the main constraint for rice production in rainfed ecologies. Low relative water content (RWC) at
reproductive stage is the most important limiting factor for
the reduction of yield and its parameters. Identifying QTLs
linked to RWC at reproductive stage can provide more
opportunity to the researchers in maker-assisted breeding
approach. For QTL associated to RWC in rice, two
contrasting parents CR 143-2-2 and Krishnahamsa for
drought tolerance were utilized for the development of
recombinant inbred lines (RILs). Significant variation for
RWC was observed among the RILs and parental lines in
both the years of study. Bulked segregant analysis was
utilized for genotyping of the mapping population. Two
hundred and one SSR markers were selected and tested for
parental polymorphic analysis from which 77 polymorphic
markers were used for genotyping the RILs. Inclusive
composite interval mapping detected, qRWC9.1 with LOD
value of 4.27 and phenotypic variation (PVE%) of 60.87 within
the marker interval of RM316-RM257 on chromosome 9.
Consistent effect of this RWC QTL for drought tolerance
traits was detected in both the years with the same marker
interval. Hence, this QTL may be useful for marker-assisted
improvement at reproductive stage drought tolerance in
rice.
 

Keywords: Drought, molecular mapping, relative water content, novel QTL

Info

Year: 2018
Volume: 78
Issue: 4
Article DOI: 10.31742/IJGPB.78.4.1
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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