Indian Society of Genetics & Plant Breeding

Protoplast studies in cotton (Gossypium Spp.)

Published on

Different explants like tender leaves, cotyledonary leaves,
hypocotyl and hypocotyl derived callus of G. hirsutum
cv. Abadhita and G. herbaceum cv. Jayadhar were used
for protoplast isolation. Protoplasts were isolated in the
enzyme mixture consisting of 5 per cent cellulase, 5 per
cent pectinase, 7.5 per cent mannitol and inorganic salts
(pH-5.S). Cotton protoplasts originating from different
explants varied in size and shape. The variation in size
was more evident in callus derived protoplasts. Callus
derived protoplast yield was highest followed by tender
leaves and ieast in hypocotyl segments. Protoplast
viability test based on Evan's blue staining indicated that
per cent of nonviable protoplasts was more from hypocotyl
explants followed by cotyledonary leaf and least in callus.
Protoplasts derived from different explants did not show
any cell division but callus derived protoplasts cultured
on MS liquid medium +2.5 gil glucose+O.1 mgll NAA
developed cell wall and microcolonies after 12-15 days
of incubation.

Keywords: Cotton, protoplast, explant, hypocotyl, cotyledon, callus and viability


Year: 2002
Volume: 62
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



Download PDF