Indian Society of Genetics & Plant Breeding

Protoplast isolation, culture and plant regeneration in Butterflypea [Clitoria ternatea (Linn.)]

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This study describes successful plant regeneration using
protoplasts isolated from mesophyll cells of Clltoria
ternatea (Linn.). Maximum protoplast yield (7.34 x 106
g/fresh wt of leaf tissue), and viability (86.3%) was recorded
with an enzyme mixture containing 1.0% (wlv) Cellulase,
1.5% (w/v) Macerozyme and 0.3% (w/v) Pectolyase. An
optimum density of 2.5 x 103 protoplasts/ml was required
for the highest frequency (71.25 ± 6.85%) of division.
Dividing protoplasts produced microcalli that proliferated
on MS medium containing 9.29 pM Kinetin and produced
shoot buds when MS medium incorporated with 100 mg
1-1 adenine sulphate. Elongated shoots were rooted on
half strength MS basal medium without growth regulators.
The well rooted shoots were transferred and acclimatized.

Keywords: Cliloria, Plant regeneration. Protoplasts


Year: 2003
Volume: 63
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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