Indian Society of Genetics & Plant Breeding

Polyethyleneglycol mediated rapid in vitro screening of rice (Oryza sativa L.) genotypes for drought tolerance

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Cultivation of tolerant genotypes in drought prone areas is
the right option to augment productivity of rice. Thus,
rapid, feasible and cost effective in vitro protocol for
screening a large number of genotypes was standardized.
MS medium fortified with 80 mg/L of PEG was found to be
the best concentration for screening under in vitro drought
situation. Tolerance index was used to classify the
genotypes. Based on tolerance index of the individual
seedling parameter, varieties like Annada, IET 24171, MTU
1010, Bitti-1 and Tulsibhog were classified as tolerant.
Similarly, judging the mean rank score of all the seedling
parameters of the genotypes IET 24171, Annada, MTU 1010,
Malsira, Tulsibhog and Bitti-1 were categorized as tolerant.
In these two methods of classification, IET 24171, Annada,
Bitti-1, MTU 1010 and Tulsibhog were common.

Keywords: Rice cultivars, in vitro screening, drought tolerance

Info

Year: 2018
Volume: 78
Issue: 1
Article DOI: 10.5958/0975-6906.2018.00018.4
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle
Bidhan Roy info_circle
T. S. Ghimiray info_circle

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