Indian Society of Genetics & Plant Breeding

Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.)

Published on

Efficient plant regeneration via somatic embryogenesis
has been developed in chickpea from embryonic axes on
MS medium supplemented wth 1.25 mgll 2, 4-0 and 0.25
mgn, kinetin induced callusing after 7 days of culture.
On the same medium, 7 days old callus masses started
differentiating with high efficiency of globular structures
during dark incubation. 1/2 MS salts + B5 vitamins + 40
gil, sucrose was found suitable for embryo maturation.
The well formed embryos germinated into plantlets on
MS salts + B5 vitamins + 1.25 mgll IBA + 2.0 mgll BAP.
Normal plants were transferred to soil.

Keywords: Chickpea, in vitro regeneration, somatic embryogenesis, explants


Year: 2002
Volume: 62
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



Download PDF