Indian Society of Genetics & Plant Breeding

Plant regeneration from hypocotyls of black carrot via direct somatic embryogenesis and determination of its genetic stability by RAPD and iPBS methods

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A study was carried out to investigate plant regeneration
from hypocotyls of black carrot (Daucus carota spp. sativus),
an industrially and medicinally important plant via somatic
embryogenesis, and the genetic stability of obtained
plantlets with the random amplified polymorphic DNA
(RAPD) and inter primer binding sites (iPBS) methods.
Hypocotyl explants isolated from in vitro germinated seeds
were incubated initially at Murashige and Skoog (MS)
medium containing 0.5 mg/L or 1 mg/L 2,4-D for 2 weeks
and then at hormone-free MS medium in order to obtain
somatic embryos, which were observed after 15 days on
the explants. Performed molecular analyses showed that
all bands obtained from plantlets through micropropagation were monomorphic. This protocol where black
carrot was determined to be clonally reproduced through
somatic embryogenesis may lead the way for further
research regarding germplasm conservation and breeding

Keywords: Black Carrot, direct somatic embryogenesis, micropropagation, molecular markers, plant growth regulators


Year: 2018
Volume: 78
Issue: 3
Article DOI: 10.31742/IJGPB.78.3.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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